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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Endocr Relat Cancer. 2014 Sep 16;21(6):865–877. doi: 10.1530/ERC-14-0424

FIGURE 4.

FIGURE 4

PAK activity is not MEK-dependent. A. Western Blot of protein isolated from BCPAP, TPC1 and FTC133 cell lines treated with U0126 (or control DMSO) at doses sufficient to inhibit MEK kinase function did not alter PAK-mediated phosphorylation of vimentin at serine 56. B. 2.0 µM of U0126 reduced thyroid cancer cell migration compared to DSMO control. Statistical significance (p values) as noted in the figure. C. MTT assays confirmed that 2.0 µM of U0126 in the conditions used for migration did not reduce cell viability. WB demonstrates that a reduction of MEK1/2 levels with siRNA also did not affect pPAK or PAK1 protein levels versus cells transfected scrambled sequence control. Western blots are representative of experiments performed on at least three independent occasions.