miR-153 reduces the number of migrated and invaded PDAC cells. (A) MIAPaCa-2 cells that were transfected with miR-control (control) and miR-153, respectively, were subjected to RT-qPCR for miR-153 expression. n, three independent experiments, *P<0.05. (B) Cell migration as measured by Boyden chamber assays was inhibited by the upregulation of miR-153 in MIAPaCa-2 cells as compared with the control cells. miR-153-upregulated MIAPaCa-2 cells conferred a fewer number of invaded cells as compared with control cells. n, three independent experiments, *P<0.05. (C) AsPC-1 cells that were transfected with negative control (NC) and miR-153 inhibitor (anti-miR-153), respectively, were subjected to RT-qPCR for miR-153 expression. n, three independent experiments, *P<0.05. (D) Downregulation of miR-153 promoted AsPC-1 cell migration and invasion in vitro. n, three independent experiments, *P<0.05. PDAC, pancreatic ductal adenocarcinoma.