Figure 4.

SNAI1 is identified as a functional target of miR-153 in PDAC. (A) RT-qPCR and (B) western blot analysis of SNAI1 expression in MIAPaCa-2 cells with miR-153 or control vector transfection. n, three independent experiments, ^*P<0.05. (C) miR-153 and its putative binding sequence in the 3′-UTR of SNAI1. The mutant miR-153 binding site was generated in the complementary site for the seed region of miR-153 (wt, wild-type; mt, mutant type). (D) miR-153 significantly suppressed the luciferase activity that carried wt but not mt 3′-UTR of SNAI1. Anti-miR-153 led to a marked increase in luciferase activity of wt 3′-UTR of SNAI1. n, three repeats with similar results, ^*P<0.05. PDAC, pancreatic ductal adenocarcinoma; 3′-UTR, 3′-untranslated region.