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Polystyrene single molecules immobilized on silicon wafer treated with a solution of ODTMS/PFPA-silane at the molar ratio of a) 500:1, b) 2000:1, and c) 5000:1. Monodisperse polystyrene was used; Mw 223 200. The scan area was 1 µm × 1 µm for all images.
