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Proper preparation of an anaesthetized lizard for perfusion. (A) The lizard is pinned abdomen-up to a dissection pad (step 4.2). (B) A horizontal incision is made just below the sternum (step 4.3). (C) Two parallel incisions are made up from the horizontal incision to the clavicle (step 4.4). (D) the abdomen is lifted, inverted, and pinned to the dissection pad, exposing the heart (step 4.5).
