Figure 1.

Some GABAergic cells selectively express COUP-TFII in the dorsal hippocampus. A, C, Immunolabeling for COUP-TFII shows neuronal nuclei immunopositive for horseradish peroxidase product in all layers (arrows) at two magnifications. B, D, Expression of mRNA for GAD as detected by an antisense radioactive riboprobe recognizing both GAD-65 and GAD-67 isoforms. Note varying levels of expression of both COUP-TFII and GAD mRNA in different cells. Small immunolabeled nuclei (arrowheads in C, D) are perivascular cells that do not express GAD. The white matter labeling at top of A, B and upper left of C, D is not specific immunoreactivity. SO, stratum oriens; SP, stratum pyramidale; SR, stratum radiatum; SLM, stratum lacunosum-moleculare. Scale bars: (A, C), 100 μm.