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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Sep 28;1843(12):3029–3037. doi: 10.1016/j.bbamcr.2014.09.017

Figure 1. Predominantly cytosolic and modest nuclear localization of exogenously expressed Slfn3 mimics that of the endogenous molecule in IEC-6 cells.

Figure 1

(A) Slfn3 protein abundance is greatest in the cytosol in fractionated rat IEC-6. Bars represent densitometric analysis as a ratio of the total immunoreactivity (*p<0.05, n=8). A representative gel is shown above; Rho GDI and Lamin B were used as cytosolic and nuclear markers. (B) Confocal images confirm that endogenous immunoreactive Slfn3 is found in the nucleus but is predominantly localized to the cytosol: (a) Slfn3 primary antibody/FITC secondary antibody, (b) DAPI nuclear stain, (c) merged image, and (d) no primary antibody control with DAPI stain. Representative of 8 similar images. (C) (a) Exogenously expressed Slfn3 (pEGFP-Slfn3-C1) and (b) endogenous Slfn3 co-localize in IEC-6 cell (c). Cytosolic/nuclear expression is further delineated with nuclear DAPI staining (b, d, f). Representative of 6 similar.