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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Biochem J. 2015 Jun 1;468(2):345–352. doi: 10.1042/BJ20150030

Figure 1. Nuclear localization of CHD5: Western analysis.

Figure 1

Various NB cell lines were subjected to SDS/PAGE (4 %–12% gels) and probed with fraction-specific antibodies as shown on the right. (A) Subcellular fractions, cytosol, membrane, nuclear (Nuc) and chromatin-bound nuclear (Nuc2), for NLF and NLF–CHD5. (B) Cellular fractions, cytosol and nuclear, for NLF, NLF–CHD5, NBLS and NY5Y. CHD5 (∼250 kDa) was detected only in the nuclear fraction from NLF–CHD5-S, NBLS and SY5Y (very low expression), but not in cytosolic or membrane fractions. CHD4 was present in all nuclear fractions as expected in all tested cell lines. Blots were stripped and probed with GAPDH and p65, the larger subunit of NF-kB, as markers for cytosolic fractions.