Table 1. IP/MS identified known canonical NuRD components.

Nuclear extracts expressing CHD5 were immunoprecipitated with CHD5 (A) or CHD4 (B) antibodies independently followed by SDS/PAGE analysis. Gels were stained with Simply Blue and excised gel bands were sent for MS analysis. MS analysis revealed the list of known canonical NuRD components as shown below. Total, number of total peptides present in a given sample application; Unique, number of peptides that are unique and specific to the indicated gene/protein description.

A. CHD5–NuRD
Gene symbol	Description	Unique	Total
CHD5	Chromodomain helicase DNA-binding protein 5	56	97
MTA1	Metastasis-associated 1	18	21
MTA2	Metastasis-associated 2	27	34
GATAD2A/p66α	GATA zinc finger domain containing 2A	23	41
GATAD2B/p66β	GATA zinc finger domain containing 2B	15	26
HDAC1	Histone deacetylase 1	11	16
HDAC2	Histone deacetylase 2	7	11
RBBP4/RbAp48	Retinoblastoma-binding protein 4	14	20
RBBP7/RbAp46	Retinoblastoma-binding protein 7	4	5
MBD2	Methyl-CpG-binding domain protein 2	4	4
MBD3	Methyl-CpG-binding domain protein 3	7	8
B. CHD4–NuRD
Gene symbol	Description	Unique	Total
CHD4	Chromodomain helicase DNA-binding protein 4	91	275
MTA1	Metastasis-associated 1	20	30
MTA2	Metastasis-associated 2	28	45
GATAD2A/p66α	GATA zinc finger domain containing 2A	27	77
GATAD2B/p66β	GATA zinc finger domain containing 2B	25	37
HDAC1	Histone deacetylase 1	15	28
HDAC2	Histone deacetylase 2	10	26
RBBP4/RbAp48	Retinoblastoma-binding protein 4	18	42
RBBP7/RbAp46	Retinoblastoma-binding protein 7	5	6
MBD2	Methyl-CpG-binding domain protein 2	7	8
MBD3	Methyl-CpG-binding domain protein 3	9	14