Table 1.
Porcine and mouse primers for real-time reverse transcription-polymerase chain reaction
| Gene name | Species | 5′ ← DNA sequence → 3′ | Product size | Accession number | |
|---|---|---|---|---|---|
| CXCL14 | Porcine | Forward | ACGGGTCCAAGTGAAGTGC | 206 bp | AY308800 |
| Reverse | GGCGTTGTACCACTTGATGA | ||||
| G-CSF | Porcine | Forward | CTGGACTGGGAGGTAGTTG | 97 bp | NM_213842 |
| Reverse | CTTAGGGTAGGGGTTCACTC | ||||
| BDNF | Porcine | Forward | TTCAAGAGGCCTGACATCGT | 180 bp | NM_214259 |
| Reverse | AGAAGAGGAGGCTCCAAAGG | ||||
| NPY | Porcine | Forward | CTCCGCCTCTGATAGTCTGC | 200 bp | NM_001256367 |
| Reverse | CACTTCCCATCACCACACAG | ||||
| IL-1-a | Porcine | Forward | CAAGGACAGTGTGGTGATGG | 215 bp | NM_214029 |
| Reverse | CATCATTCAGGATGCAGTGG | ||||
| IL-6 | Porcine | Forward | AGCAAGGAGGTACTGGCAGA | 187 bp | NM_214399 |
| Reverse | CAGCCTCGACATTTCCCTTA | ||||
| IL-8 | Porcine | Forward | GCATTCCACACCTTTCCAC | 145 bp | NM_213867 |
| Reverse | CTTCTGCACCCACTTTTCC | ||||
| IL-16 | Porcine | Forward | TCAGCAGCCAAGTGTCATC | 197 bp | NM_213751 |
| Reverse | TCTCAGCTCCGAAAGGTTG | ||||
| MCP1 | Porcine | Forward | TCTCCAGTCACCTGCTGCTA | 185 bp | NM_214214 |
| Reverse | TCCAGGTGGCTTATGGAGTC | ||||
| β-actin | Porcine | Forward | CTCTTCCAGCCCTCCTTCCT | 80 bp | AJ312193 |
| Reverse | ACGTCGCACTTCATGATCGA | ||||
| Syndecan 3 | Mouse | Forward | TGGCTACCTTGGACACAGAG | 181 bp | NM_011520 |
| Reverse | GACTCTGGAGTTGGGGTCTG | ||||
| TRH-DE | Mouse | Forward | CCAGCAGGCATCAACACTTA | 205 bp | NM_146241 |
| Reverse | CCTGTCATCACTGCAAGTTA | ||||
| CXCR4 | Mouse | Forward | CCATGGCTGACTGGTACTTT | 210 bp | NM_009911 |
| Reverse | TCAGGAGGAGGGCTGGGATC | ||||
| CCR2 | Mouse | Forward | ATTCTGGGCTCACTATGCTG | 188 bp | NM_009915 |
| Reverse | TCACCCCAAAGGTAACTGTC | ||||
| β-actin | Mouse | Forward | AAGTACCCCATTGAACACGG | 257 bp | NM_007393 |
| Reverse | ATCACAATGCCAGTGGTACG |
BDNF, Brain-derived neurotrophic factor; bp, Base pairs; CCR2, Chemokine (C-C motif) receptor 2; CXCL14, Chemokine (C-X-C motif) ligand 14; CXCR4, Chemokine (C-X-C motif) receptor 4; G-CSF, Granulocyte colony-stimulating factor; IL, Interleukin; MCP1 (CCL2), Monocyte chemotactic protein 1 (chemokine (C-C motif) ligand 2); NPY, Neuropeptide Y; TRH-DE, Thyrotropin-releasing hormone-degrading enzyme