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. 2015 Jun 29;10(6):e0131928. doi: 10.1371/journal.pone.0131928

Fig 3.

Fig 3

(A) Comparison of the relative level of yebK mRNA in strains OSS, OSS-yebK*, OSS-ascB*, and ESS grown on cellobiose minimal medium. MG1655 RNA was prepared from cells grown on glucose minimal medium. Error bars indicate the standard deviation of experiments performed in triplicate. Statistical analysis was performed using one-way ANOVA with MG1655 grown on glucose minimal medium as control. Asterisk indicates the statistically significant comparison with p<0.05. The p value was marked for one indicative strain grown on cellobiose compared to the wild type grown on glucose. Statistical analysis indicates that difference in yebK expression-level was significant between glucose and cellobiose grown cells but not among different strains growing on cellobiose. (B) EMSA to analyze the auto-regulatory role of 6 His-YebK. The promoter of yebK was incubated with different concentration of purified 6 His-YebK protein in the presence or absence of KDPG and analyzed on 5% non-denaturing polyacrylamide gel. (C) Growth of edd gene deleted strains on cellobiose minimal medium. OSS—closed diamond; OSS-yebK*–closed triangle; OSS-Δeda–open diamond; OSS-yebK*/Δeda–open triangle; OSS-ascB*–closed square. Error bars indicate the standard deviation of experiments performed in triplicate.