|
Strains
|
|
|
|
E. coli MG1655 |
Wild type |
[23] |
| OSS |
MG1655 with chb and asc operon promoters replaced with CP12 |
[6] |
| ESS |
OSS adapted in cellobiose for 30 days |
[6] |
| OSS-yebK* |
OSS with nonsense mutation in yebK
|
This study |
| OSS-ascB* |
OSS with 10-nt inserted in the RBS region of ascB
|
This study |
| OSS-yebK*/ascB* |
OSS with both mutations in ESS |
This study |
| MG1655/ΔPasc-ascB::frt |
MG1655 with PascG to ascB deleted |
This study |
| MG1655/ΔchbB- chbF::frt |
MG1655 with chbB to chbF deleted |
This study |
| OSS-Δeda::frt
|
OSS with eda gene deleted |
This study |
| OSS-Δedd::frt
|
OSS with edd gene deleted |
This study |
| OSS-yebK*/Δedd::frt
|
OSS with nonsense mutation in yebK and eddgene deleted |
This study |
| OSS- P2E5 |
OSS with RBS of ascB mutated through MAGE |
This study |
|
Plasmids
|
|
|
| pSIM5 |
λ-Red recombinase expression plasmid and temperature-sensitive replication |
[24] |
| pCP20 |
Yeast FLP recombinase gene controlled by CI repressor and temperature sensitive replication. |
[25] |
| pKD13 |
Template plasmid for gene disruption. The kanamycin resistance gene is flanked by FRT sites. oriR6K requiring the pir+ E. coli. |
[7] |
| pKD13-SacB |
Modified form of pKD13 with kanamycin gene co-expressed with sacB gene |
This study |
| pET31b+yebK-6his |
pET31b+ plasmid with yebK-6 His epitope tag |
This study |
| pET31b+yebK*-6his |
pET31b+ plasmid with yebK*-6 His epitope tag |
This study |
| pProbe-NT' |
pBBR1 ori., KmR
|
[26] |
| pProbe-A5 |
pProbe-NT' plasmid containing the sequence + 391 nt from the start codon of ascF till the end of ascB cloned into the EcoRI, KpnI site |
This study |
