Fig. 2.

Detection of differentiated 3T3-L1 adipocytes. 3T3-L1 Preadipocytes were maintained in growth medium (Undifferentiated), or induced for adipocyte differentiation in the absence (Differentiated control) or presence of various concentrations of 1 or rosiglitazone. Differentiated adipocytes were stained red by oil red O. Pictures were taken under an inverted microscope (×100). Absorption of Oil red O for each treatment was measured at 492 nm (A_492nm) after dissolving the cellular Oil red O with isopropanol, differentiation rate (DR) was calculated by equation DR (%) = 100 × (A_Sample − A_{Undifferentiated})/(A_{Differentiated control} − A_{Undifferentiated}). *P < 0.05, ***P < 0.001 versus Differentiated control