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. 2015 Jun 17;2015:165867. doi: 10.1155/2015/165867

Figure 2.

Figure 2

Effects of serum depletion on cell characteristics of A549 clones. (a) Growth curve of B7 (left) and H12 (right). Black: cultured with serum, red: cultured without serum. (b) and (c) Expression and quantitative analyses of SPC, AQP5, and HIF-1α mRNA in B7 cells (b) and in H12 cells (c). (Upper panel) representative RT-PCR results. N1: negative control without RT reaction, N2: negative control with distilled water, and P: positive control with sequenced PCR products in plasmid. (Lower three panels) quantitative analysis of gene expression. Black: cultured with serum, red: cultured without serum. Experiments were independently performed in triplicate. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.