Table 1.
Primers used for RT-PCR.
| Gene | Primer | Annealing (°C) | Cycle | Expressed cells |
|---|---|---|---|---|
| FLK | F: 5′-AAGGCTCAAACCAGACAAGC-3′ R: 5′-TTCCTGCTGGTGGAAAGAAC-3′ |
58 | 30 | Early mesoderm |
| PDGFRα | F: 5′-GGGGAGAGTGAAGTGAGCTG-3′ R: 5′-AGTCTCGGGATCAGTTGTGC-3′ |
58 | 30 | |
|
| ||||
| FOXA2 | F: 5′-CCGGATCGAGGACAAGTGAG-3′ R: 5′-CAACAACAGCAATGGAGGAG-3′ |
58 | 30 | Endoderm |
| SOX17 | F: 5′-AAGGGCGAGTCCCGTATCC-3′ R: 5′-TTGTAGTTGGGGTGGTCCTG-3′ |
58 | 30 | |
|
| ||||
| TTF-1 | F: 5′-ACCAGGACACCATGAGGAAC-3′ R: 5′-GCGCCGACAGGTACTTCTG-3′ |
58 | 30, 40 | Lung progenitor |
|
| ||||
| p63 | F: 5′-TTGCCCCTCCTAGTCATTTG-3′ R: 5′-TACTGTCCGAAACTTGCTGC-3′ |
58 | 33 | Basal |
|
| ||||
| FOXJ1 | F: 5′-TGGATCACGGACAACTTCTG-3′ R: 5′-AAGTTGCCTTTGAGGGGTTC-3′ |
58 | 40 | Ciliated |
|
| ||||
| MUC5AC | F: 5′-TACGTGTTCTCCGAGCACTG-3′ R: 5′-GGTTCCACATGAGGACAAGG-3′ |
58 | 30 | Goblet |
|
| ||||
| CC10 | F: 5′-GTCACACTGGCTCTCTGCTG-3′ R: 5′-GAGCAGTTGGGGATCTTCAG-3′ |
58 | 40 | Clara |
|
| ||||
| AQP5 | F: 5′-ATCTTCGCCTCCACTGACTC-3′ R: 5′-TTTCTTCTTTTCCCCCTTGG-3′ |
56 | 40 | Alveolar type I |
| T1α | F: 5′-TGGGGTCTTACTAGCCATCG-3′ R: 5′-TAGAGGAGCCAAGTCTGGTG-3′ |
58 | 40 | |
|
| ||||
| CFTR | F: 5′-GCCAGCGTTGTCTCCAAAC-3′ R: 5′-CGATAGAGCGTTCCTCCTTG-3′ |
58 | 30 | Alveolar type II |
| SPB | F: 5′-AAGTTCCTGGAGCAGGAGTG-3′ R: 5′-AGAGGAATGGGGAATTGCTG-3′ |
58 | 40 | |
| SPC1 | F: 5′-TTGGTCCTTCACCTCTGTCC-3′ R: 5′-CTCCCACAATCACCACGAC-3′ |
58 | 40 | |
| SPC2 | F: 5′-AACGCCTTCTTATCGTGGTG-3′ R: 5′-AAGACTGGGGATGCTCTCTG-3′ |
58 | 35 | |
|
| ||||
| CD133 | F: 5′-GCAGGGATTATTCTATGCTGTG-3′ R: 5′-ACGCCTTGTCCTTGGTAGTG-3′ |
58 | 35 | Cancer stem |
|
| ||||
| HIF-1α | F: 5′-TGCTCATCAGTTGCCACTTC-3′ R: 5′-TCTCATTTCCTCATGGTCAC-3′ |
56 | 30 | Cancer |
|
| ||||
| 18SrRNA | F: 5′-TACCTGGTTGATCCTGCCAGTAGGAT-3′ R: 5′-CCCGTCGGCATGTATTAGCTCTAGAA-3′ |
58 | 17 | |