Table 2.
Cell type | Early mesoderm | Endoderm | Lung progenitor cells | Basal cells | Ciliated cells | Goblet cells | Clara cells | Alveolar type I cells | Alveolar type II cells | Cancer stem cells | ||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Days of cultures | Marker genes | |||||||||||||
FLK | PDGFRα | SOX17 | FOXA2 | TTF-1 | p63 | FOXJ1 | MUC5AC | CC10 | T1α | AQP5 | SPC | CFTR | CD133 | |
| ||||||||||||||
d1 | — | — | — | + | — | + | + | + | + | — | + | + | — | + |
d2 | — | — | — | + | — | + | + | + | + | — | — | + | — | + |
d3 | — | — | — | + | — | + | + | + | + | — | — | + | — | + |
d4 | — | — | — | + | — | + | + | + | — | — | — | + | — | ++ |
RT-PCR results shown in Figure 1(b) were quantified and normalized by ribosomal 18S. The relative ratio of each gene expression level was evaluated by the lowest expression level among the samples as 1. —: not detected; +: 1–2.5-fold; ++: over 2.5-fold.