Table 3.

Optical analyses of A549 clones.

A549 clones			Characteristics of cloned cells
Group number	Plate A	Plate B	Days to confluent	Cell thickness	Cell morphology	Boundary of cell cluster	Cell density	Clusterius	Others
(1)	E2, E5, H9, H10	A2, A3, B2, C6, F5, F9, G1, G2, H10, H11	3.5	Thin	Spindle	Unclear	Low	Low
(2)		C10, C11	4	Thin	Polygonal	Clear	High	High
(3)	C5, E8	A8, D12, G5	3.5	Thin	Polygonal	Clear	Low	High
(4)	G8		14	Thick	Polygonal	Unclear	Low	High	Piling up, granule-rich
(5)		A10, E12	3.5	Thin	Polygonal	Unclear	Low	Low
(6)	B7, F5	C2, C4, C12, G8	3.5	Thick	Spindle	Unclear	Low	Low
(7)	F8, H12		4.5	Thick	Polygonal	Clear	High	High
(8)		B6, B7, B10, C1	4	Thick	Polygonal	Unclear	High	Low
(9)	A12		3.5	Thick	Polygonal	Unclear	Low	Low
(10)		D2	3	Thick	Polygonal	Unclear	Low	Low	Granule-rich
(11)	H11	A5, B3, C8, D7, G7	3.5	Thick	Polygonal	Unclear	Low	Low
(12)	G9-1, G9-2		4.5	Thick	Invadopodia	Unclear	Low	Low	Bright cell body

Optical observations of isolated 46 clones were summarized and categorized into 12 groups. Cell thickness, mean gray value ≥5: thick, <5: thin. Boundary of cell cluster: smoothly draw the outline of cluster: clear; hard to draw the outline: unclear. Cell density, the number of cells in the frame of 100 μm × 100 μm ≥10: high, <10: low. Clustering: the number of cell cluster ≥3: high, <3: low.