Table 4.

Gene expression patterns in A549 clones.

Cell type	Early mesoderm		Endoderm		Lung progenitor cells	Basal cells	Ciliated cells	Goblet cells	Clara cells	Alveolar type I cells		Alveolar type II cells		Cancer stem cells	Summary of gene expression patterns	Class
A549 clone	FLK	PDGFRα	SOX17	FOXA2	TTF-1	p63	FOXJ1	MUC5AC	CC10	T1α	AQP5	SPC	CFTR	CD133
(1) H10	—	—	—	+	—	+	+	++++	+	—	—	—	—	++	Nontypical	1
(2) C11	—	—	—	+	—	+	+	++	+	—	+	+	—	+	ATI ATII	3
(3) C5	—	—	—	+	—	+	—	+	—	—	—	+	—	++	ATII	4
(4) G8	—	+	—	—	—	+	—	—	—	—	—	++	—	—	ATII, no FOXA2 CD133	2
(5) A10	—	—	—	+	—	+	—	+++	—	—	+	+	—	+	ATI ATII	3
(6) C2	—	—	—	+	—	+	++	+++	—	—	—	+	—	+	ATII	4
(7) H12	—	—	—	+	—	+	+++	++++	—	—	++	+	—	++	ATI ATII	3
(8) B7	—	—	—	+	—	+	+++	++	—	—	—	+	—	+	ATII	4
(9) A12	—	—	—	+	—	+	++	+++	—	—	—	+	—	+	ATII	4
(10) D2	—	—	—	+	—	+	+	++++	—	—	+	+	—	+	ATI ATII	3
(11) D7	—	—	—	+	—	+	+	++++	—	—	+	+	—	+	ATI ATII	3
(12) G9	—	—	—	+	—	+	+	++++	—	—	—	+	—	+	ATII	5

RT-PCR results shown in Figure 1(c) were quantified and normalized by ribosomal 18S. The evaluation and indications were the same as in Table 2. —: not detected; +: 1-2-fold; ++: 2-3-fold; +++: 3-4-fold; ++++: 4-5-fold. On the basis of the gene expression patterns and optical features, 12 groups of clones were further categorized into 5 classes of cell characters were selected in this study. Class 1: H10, class 2: G8, class 3: C11, A10, H12, D2, and D7, class 4: C5, C2, B7, and A12, and class 5: G9.