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Whole cell extracts were sequentially immunoprecipitated using an antibody against TbP34/P37 and then an antibody against TcL5 to enrich the precipitate in complexes containing both components. Reverse transcription was then performed on these complexes to specifically detect the presence of 5S rRNA. Beads: beads alone (no antibody),-RT: no reverse transcriptase, +RT: reverse transcriptase included.
