Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jun 29;10(6):e0131323. doi: 10.1371/journal.pone.0131323

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Umaer, Williams

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Fig 6 — Panel A. Purification of recombinant TcP37/NRBD and TcL5. Coomassie blue staining of SDS-PAGE of eluted fractions (E1-E4) from a Ni-NTA affinity column. M: Molecular weight markers (in kDa). Panel B: Recombinant TcP37/NRBD and TcL5 bind in vitro transcribed Tc 5S rRNA. The filter binding assay using radiolabeled T. cruzi 5S rRNA and rTcP37/NRBD and rTcL5. The percentage of signal bound to the filters was plotted against the concentration of recombinant protein in each reaction. The line represents the fit to a bimolecular equilibration calculated in Graphpad Prism 5.