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. 2015 Jun 29;10(6):e0129851. doi: 10.1371/journal.pone.0129851

Fig 5. Resibufogenin down-regulates cyclin D1 through proteasomal degradation.

Fig 5

(A) The effect of MG132 on suppression of cyclin D1 expression by resibufogenin in HT-29 cells. Cells were treated by 5 μM resibufogenin with or without 20 μM MG132 for 24 h. (B) The effect of MG132 on suppression of cyclin D1 expression by resibufogenin in A549 cells. Cells were treated with 2.5 μM resibufogenin with or without 20 μM MG132 for 24 h. (C) The effect of SB216763 on suppression of cyclin D1 expression by resibufogenin in HT-29 cells. Cells were treated by 5 μM resibufogenin with or without 30 μM SB216763 for 3 h. (D) The effect of SB216763 on suppression of cyclin D1 expression by resibufogenin in A549 cells. Cells were treated with 2.5 μM resibufogenin with or without 30 μM SB216763 for 6 h. In all figures, GAPDH was used as a loading control for protein quantitation. UT, untreated; DM, treated with DMSO. The band intensity was measured and normalized by GAPDH, and the protein levels are shown at the bottom of each blot.