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. 2015 Jun 29;10(6):e0131680. doi: 10.1371/journal.pone.0131680

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© 2015 Liang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — Spleen and thymus were harvested 24 h after CLP. A. Representative sections analyzed using in situ TUNEL assays in spleen. Apoptotic cells are stained with a yellow-brown color in the nuclei (red arrows). B. The percentage of TUNEL-positive cells represents apoptotic cells in spleen. C. Flow cytometry dot plots of annexin V/PI staining. D. The percentage of FACS-positive cells represents apoptotic cells in thymus. Apoptosis was calculated as the percentage of annexin V-positive cells that were the sum of the percentage of cells included in the lower and upper right quadrants. Bars represent the mean ±SD (n = 6 each group). **p <0.01, *p <0.05 vs. sham; # p <0.05 vs. CLP.