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Blood, thymus, and spleen were harvested 24 h after CLP. For spleen and thymus, single-cell suspensions were prepared and the total cell number was counted after erythrocyte lysis. Using FACS analyses, lymphocyte numbers were acquired by the total number and percent of lymphocyte subgroup. **p <0.01, *p <0.05 vs. sham; # p <0.05 vs. CLP.
