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. 2015 Jun 29;10(6):e0130416. doi: 10.1371/journal.pone.0130416

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© 2015 Singer et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — A. Cellular localization of transiently transfected EGFP- (N-terminal) and DsRed2- (C-terminal) tagged NLRP7 or ZBTB16 in HEK293T cells, respectively. Nucleus was counterstained with ToPro3. ZBTB16 localizes in the nuclear speckles, while NLRP7 is distributed in the cytoplasm with occasional accumulation near the nucleus. B. Upper row: Co-localization of transiently transfected N-terminal EGFP-tagged NLRP7 and C-terminal DsRed2-tagged ZBTB16 in HEK293T cells. Both proteins co-localize in the cytoplasm near the nucleus. ZBTB16 is re-localized from the nucleus (stained with ToPro3) to the cytoplasm. Lower row: Co-localization of transiently transfected C-terminal DsRed2-tagged NLRP7 and N-terminal EGFP-tagged ZBTB16 in HEK293T. Again, both proteins co-localize in the cytoplasm in a juxtanuclear aggregate while some amounts of EGFP-tagged ZBTB16 remain in the nucleus (stained with ToPro3). C. Single left panel: Staining of endogenous ZBTB16 in non-transfected HEK293T cells. The protein shows its typical nuclear localization in the nuclear speckles. Right row: Transient transfection of N-terminal EGFP-tagged NLRP7 in HEK293T cells results in nuclear export of endogenous ZBTB16 and shows their co-localization in a cytoplasmic juxtanuclear aggregate. D. Single left panel: Transient transfection of C-terminal DsRed2-tagged KHDC3L in HEK293Tcells. The protein shows a diffuse distribution in the cytoplasm with occasional accumulation near the nucleus. Right row: Transient transfection of N-terminal EGFP-tagged NLRP7 and C-terminal DsRed2-tagged KHDC3L in HEK293T cells. Both proteins co-localize diffusely in the cytoplasm and in juxtanuclear aggregates.