Fig 4. Binding of BP₁₀-T-EGF to ßTCP scaffolds and elution over 7 days.

Binding isotherms of BP ₁₀ -T-EGF for 5mm and 3mm crosses (A). Analysis of released and bound BP₁₀-T-EGF protein for the 2μM tethering condition shows that assays are robust (i.e., the combined bound and released at 7 days is statistically indistinguishable from the initial amount) and that 75% of the BP₁₀-T-EGF fusion protein remains bound to the scaffold after a 7-day incubation in 1xPBS at 37^°C (B).