Fig 2. t-Darpp is responsible for increased EGFR sensitivity in SK.Her^R cells.

SK-Br-3 and SK.Her^R cells were transfected with scrambled (-) or tDp siRNA (+). 24 hours later, cells were treated with DMSO or AG1478 for an additional 24 hours. (A) Western analysis to confirm siRNA knockdown. (B) SRB cell proliferation assay to determine relative sensitivity to AG1478. Shown are the averages (± S.D.) of four experiments, with normalization to DMSO-treated controls in all cases. **, p<0.01.