Table 1. Primer sequences designed for ORs in RT-PCR, real-time quantitative PCR (RT-qPCR), 3’RACE, dsRNA synthesis, the reference gene SlituRPL8 in RT-qPCR, and the control GFP in dsRNA synthesis.
Application | Gene | Forward primer | Reverse primer |
---|---|---|---|
RT-PCR | SlituOR3 | GGAATTCATGGACACCCTTCAGAAGATAT | CCAAGCTTCTATTGGATAGTAGTCTCTACACCT |
qRT-PCR | SlituOR3 | GCTGCCAAATCGGTTCATAT | AGTCTCCATCGCATGTTCAG |
SlituRPL8 | ATGCCTGTGGGTGCTATGC | TGCCTCTGTTGCTTGATGGTA | |
3’RACE | SlituOR3 | CTCTCTGCGTAATCTGGTTTTGCATGTGT | CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT |
dsRNA synthesis | SlituOR3 | TAATACGACTCACTATAGGGAGACCAC TAAACCTGGTGGCACACAAA | TAATACGACTCACTATAGGGAGACCAC GTACACGGCGCTTGGTAACT |
GFP | TAATACGACTCACTATAGGGAGATTTGTATAGTTCATCCATGCCATGT | TAATACGACTCACTATAGGGAGAATGAGTAAAGGAGAAGAACTTTTCA |