Fig 1. Cofactor and protein-only PrPSc are stably propagating recombinant PrP conformers that differ in their ability to template the conversion of native PrPC.
(A) Western blot showing three-round sPMCA reactions using recombinant PrP as the substrate and seeded with full-length or PK-digested cofactor and protein-only PrPSc, as indicated. (B) Western blot showing three-round sPMCA reactions using normal mouse brain homogenate as the substrate and seeded with full-length or PK-digested cofactor and protein-only PrPSc, as indicated.