Fig 5. Cofactor and protein-only PrP^Sc differ in their ability to template the conversion of PrP substrates containing a GPI anchor.

Western blot showing three round sPMCA reactions using partially purified and deglycosylated PrP^C as the substrate and seeded with protein-only PrP^Sc, cofactor PrP^Sc, or prion-infected brain homogenate, as indicated. A replicate of this experiment is shown in S9 Fig