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. 2015 Jun 30;10(6):e0130811. doi: 10.1371/journal.pone.0130811

Fig 2. HD5 binds specifically to cell-surface B272 homodimers expressed in human .220 B cell lines.

Fig 2

Representative flow cytometry analysis of HD5 and control antibodies (IgG1 Isotype, W6/32, ME1, HC10, and HD6) binding to LBL721.220 cells either untransfected (.220), or transfected with HLA–B7, B27.C67S (HLA-B27 with a mutation of Cys-67 to serine that abrogates cell-surface B272 expression), B27.hutpn (HLA-B27 together with transfection of human tapasin, that reduces B272 expression and increases HLA-B27 heterotrimer formation), or HLA-B27 (HLA-B27 transfected cells expressing high levels of cell-surface B272). RU = responsive units. ELISA values are expressed as mean±SEM.