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. 2015 Jun 30;10(6):e0130811. doi: 10.1371/journal.pone.0130811

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© 2015 Marroquin Belaunzaran et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — (A) Representative flow cytometry analysis of cell-surface B27₂ expression in leukocytes populations of Tg rats at different ages (6, 15, 23 and 30 weeks) from spleen and MLNs. WT leukocytes represent the control population where B27₂ is absent. (B) MFI values plotted of positive B27₂ stains from splenocytes. (C) MFI values plotted of positive B27₂ stains from MLNs. Detection of cell-surface B27₂ homodimers was performed using HD6-biotinylated and detected by streptavidin-APC. HD6 had been previously assessed as an antibody capable of recognizing cell-surface B27₂ in human [12] and rat [41] leukocyte populations. Antibody panels: CD4+ T-cells (+CD3, +CD4), CD8+ T-cells (+CD3, +CD8), NK (+CD161a,—lineage), B cells (+CD45RA,-lineage) and Monocytes (+CD172a,—RP-1).