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. 2015 Jun 30;10(6):e0130485. doi: 10.1371/journal.pone.0130485

Fig 4. APL-1 overexpression, while able to rescue partial loss of PPK-3 function, failed to rescue the ppk-3(mc46) null allele.

Fig 4

(A) Expression of APL-1::GFP failed to rescue lethality of ppk-3 null animals and failed to rescue vacuolation in apl-1::GFP; ppk-3(mc46) animals. Bar, 50μm. (B) Quantification of the relative vacuolated area in ppk-3(mc46) and apl-1::GFP; ppk-3(mc46) animals showed that APL-1 overexpression failed to rescue complete loss of PPK-3 function (n≥38, p = 0.73 (two-tailed t-test), suggesting that APL-1 functions upstream of PPK-3.