Table 4. Nucleotide substitutions in a 432-bp hypervariable region on the spaA gene of 11 E. rhusiopathiae strains during the attenuation compared with the corresponding sequence of the original strain HX130709.
| Concentration of acriflavine (% w/v) |
Number of passage |
Bacteria collected for spaA sequencing |
No. of nucleotide substitutions |
spaA type |
|---|---|---|---|---|
| 0.0025 | F1-F4 | |||
| 0.00375 | F5-F7 | F5 | 0 | b |
| 0.005 | F8-F10 | F10 | 0 | b |
| 0.0125 | F11-F12 | |||
| 0.02 | F13-F27 | F15, F20, F25 | 0 | b |
| 0.0225 | F28-F38 | F30, F35 | 0 | b |
| 0.025 | F39-F45 | F40, F45 | 0 | b |
| 0.0275 | F46-F48 | |||
| 0.03 | F49-F55 | F50, F55 | 0 | b |
| Reference strain | ||||
| HX130709 | F0 | b |