Table 2. The primer sequences of miRNAs and the 3’UTR sequences of target genes.
Item | miRNA or mRNA | Sequences |
---|---|---|
miRNA | hsa-mir-23a-3p | 65-CCUUUAGGGACCGUUACACUA-45 |
ssc-mir-23a | 62-CCUUUAGGGACCGUUACACUA-42 | |
miR23a target genes | PGC-1α 3’UTR | 3491-AGCCAUGUACUAUAAUGUGAU-3511 |
MEF2C 3’UTR | 1932-UUCCUUCUCUUGGAAUGUGAA-1952 | |
miRNA | hsa-mir-208b-3p | 67-UGUUUGGAAAACAAGCAGAAUA-46 |
ssc-mir-208b | 66-UGUUUGGAAAACAAGCAGAAUA-45 | |
miR208b target genes | Sp3 | 811-AAUAAGGUGUAUUGCGUCUUAG-831 |
Thrap1 | 551-AAAAUAUAUGUAAUCGUCUUAA-572 | |
miRNA | hsa-mir-499a-5p | 53-UUUGUAGUGACGUUCAGAAUU-33 |
ssc-mir-499-5p | 35-UUUGUAGUGACGUUCAGAAUU-15 | |
miR499 target genes | Sox6 | 1198-GAUAUAGGUAUAUAGUCUUAC-1218 |
PGC-1α peroxisome proliferator-activated receptor γ coactivator 1α, MEF2C myocyte enhancer factor 2C, Sp3 specificity protein 3, Thrap1 thyroid hormone receptor associated protein 1, Sox6 SRY-box containing gene 6.