Table 3. Sequences used for real-time PCR primers.
| Gene | Primer sequence (5' to 3') | Product size (bp) | GenBank accession no. |
|---|---|---|---|
| MyHC- I | Forward: CGTGGACTACAACATCATAGGC | ||
| Reversed: CTTTGCCCTTCTCAACAGGT | 152 | NM_213855 | |
| MyHC- IIa | Forward: AAACCTCACGGAAGAGATGG | ||
| Reversed: TCAGGGTGTTGACTTTGTCCT | 134 | NM_214136 | |
| MyHC- IIx | Forward: GAAACCGTCAAGGGTCTACG | ||
| Reversed: CGCTTCCTCAGCTTGTCTCT | 153 | NM_001104951 | |
| MyHC- IIb | Forward: GATGTTCCTGTGGATGGTCA | ||
| Reversed: CTCGTTGGTGAAGTTGATGC | 148 | NM_001123141 | |
| Sox6 | Forward: GCAGCAAGGGTCAAGAGTG | ||
| Reversed: GGCAGTTCAGGGTAAAGGTG | 130 | XM_003122960 | |
| Sp3 | Forward: CAGATGGTCAGCAGGTTCAG | ||
| Reversed: TAGCAGGAGGTGTTCCAGAG | 133 | XM_001928783.5 | |
| PGC-1α | Forward: AGGGAAGAATACCGCAGAGA | ||
| Reversed: TGTCCGTGTTGTGTCAGGTC | 135 | NM_213963 | |
| MEF2C | Forward: CGCTCTTCATCTTGGGTCAG | ||
| Reversed: CGTGTGTTGTGGGTATCTCG | 121 | NM_001044540.1 | |
| Thrap1 | Forward: CATCCCTGAAGCACACAGTC | ||
| Reversed: AACATCGGCACCCTTGATA | 130 | XM_005669005 | |
| 18s rRNA | Forward: GAGACGGTGGGACAGCG | ||
| Reversed: GCCCTCGGTCGAGTTGTC | 162 | AY265350 |
MyHC myosin heavy chain, Sox6 SRY-box containing gene 6, Sp3 specificity protein 3, PGC-1α peroxisome proliferator-activated receptor γ coactivator 1α, MEF2C myocyte enhancer factor 2C, Thrap1 thyroid hormone receptor associated protein 1.