Table 1. M. xanthus strains used in this work 1 .
Strain | Relevant characteristics | Reference |
---|---|---|
DK1622 | Wild type | [70] |
DK1217 | aglB | [71] |
DK1300 | sglG1 | [71] |
DK8615 | ΔpilQ | [72] |
DK10417 | ΔpilC | [73] |
DK10405 | Δtgl::Tc | [39] |
SA2273 | Pnat-romR-GFP (pSH1208) | [62] |
MxH2265 | ΔaglZ | [74] |
SA3377 | aglZ-yfp (pSL65*) | [75] |
SA5293 | ΔaglQ | This work |
SA5309 | Pnat-romR-GFP (pSH1208), MXAN_2541::miniHimarΩ3908 | This work |
SA5347 | Pnat-romR-GFP (pSH1208), MXAN_2540::miniHimarΩ3927 | This work |
SA3921 | ΔcglC | This work |
SA3922 | ΔgltB | This work |
SA3923 | ΔgltA | This work |
SA3924 | ΔgltC | This work |
SA5544 | Δoar | This work |
SA3935 | ΔcglC, PpilA-cglC (pDK110) | This work |
SA5911 | ΔgltB, PpilA-gltB (pDK111) | This work |
SA5912 | ΔgltA, PpilA-gltA (pDK112) | This work |
SA3938 | ΔgltC, PpilA-gltC (pDK113) | This work |
SA4452 | aglQ-mCherry | This work |
SA5209 | ΔMXAN_2542 | This work |
SA5242 | ΔcglC, Pnat-cglC (pBJA31) | This work |
SA5239 | ΔgltB, Pnat-gltB (pBJA32) | This work |
SA5228 | ΔgltA, Pnat-gltA (pBJA33) | This work |
SA5246 | ΔgltC, Pnat-gltC (pBJA34) | This work |
SA5245 | Pnat-gltB-mCherry (pBJA35) | This work |
SA5269 | ΔcglC, Pnat-gltB-mCherry (pBJA35) | This work |
SA5227 | ΔgltB, Pnat-gltB-mCherry (pBJA35) | This work |
SA5284 | ΔgltA, Pnat-gltB-mCherry (pBJA35) | This work |
SA5256 | ΔgltC, Pnat-gltB-mCherry (pBJA35) | This work |
SA5235 | Pnat-gltA-mCherry (pBJA36) | This work |
SA5261 | ΔcglC, Pnat-gltA-mCherry (pBJA36) | This work |
SA5289 | ΔgltB, Pnat-gltA-mCherry (pBJA36) | This work |
SA5229 | ΔgltA, Pnat-gltA-mCherry (pBJA36) | This work |
SA5255 | ΔgltC, Pnat-gltA-mCherry (pBJA36) | This work |
SA5247 | ΔaglZ, Pnat-gltB-mCherry (pBJA35) | This work |
SA5254 | ΔaglQ, Pnat-gltB-mCherry (pBJA35) | This work |
SA5252 | ΔaglZ, Pnat-gltA-mCherry (pBJA36) | This work |
SA5249 | ΔaglQ, Pnat-gltA-mCherry (pBJA36) | This work |
SA5243 | Pnat-gltC-mCherry (pBJA37) | This work |
SA5244 | ΔgltC, Pnat-gltC-mCherry (pBJA37) | This work |
SA5267 | ΔcglC, aglZ-yfp (pSL65*) | This work |
SA5268 | ΔgltB, aglZ-yfp (pSL65*) | This work |
SA5273 | ΔgltA, aglZ-yfp (pSL65*) | This work |
SA5259 | ΔgltC, aglZ-yfp (pSL65*) | This work |
SA5285 | ΔcglC, aglQ-mCherry | This work |
SA5257 | ΔgltB, aglQ-mCherry | This work |
SA5278 | ΔgltA, aglQ-mCherry | This work |
SA5258 | ΔgltC,aglQ-mCherry | This work |
SA5250 | Pnat-gltB-mCherry, aglZ-yfp (pBJA35, pSL65*) | This work |
SA5251 | Pnat-gltA-mCherry, aglZ-yfp (pBJA36, pSL65*) | This work |
1 Plasmids mentioned in parentheses were integrated at the chromosomal Mx8 attB site with the exception of those marked with an asterisk (*), which were integrated at the native site by a single homologous recombination event. Constructs were transcribed from the native promoter (Pnat) or the pilA promoter (PpilA) as indicated.