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. 2015 Jul 1;10(7):e0131219. doi: 10.1371/journal.pone.0131219

Fig 7. LA inhibits efficiently single and dual HIV-1/HSV-2 infection in T cells.

Fig 7

MT-4 cells were infected with HIV-1 NL4.3, HSV-2 G and HIV-1 NL4.3 + HSV-2 G for 3 days in the presence of various concentrations of LA (A), AMD3100 (B) and acyclovir (C). Cytopathicity was measured spectrophotometrically using MTS/PES (left panels). The results are normalized to untreated infected cells. Viral replication at dual infection (shown in blue) was also further investigated by flow cytometry (right panels). HIV-1 NL4.3 replication was detected with b12 mAb + RaH-FITC (white bars) and HSV-2 strain G with agB + GaM-PE (grey bars). Bars indicate the % viral replication in MT-4 cells, compared to untreated conditions. The results shown are the mean ± SEM of 3–5 independent experiments.