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. 2015 Jul 1;11(7):e1005013. doi: 10.1371/journal.ppat.1005013

Fig 3. EspC does not regulate EspA filament structures at the surface of primed bacteria.

Fig 3

EPEC strains were grown for 5 hrs in DMEM to induce T3S. (A) Epifluorescent micrographs showing EspA staining associated with bacteria primed for 30 min or 5 h in DMEM. (B) Average percentage of bacteria associated with EspA staining ± SEM, scored for at least 2900 bacteria for each sample in 3 independent experiments. The total number of analysed bacteria (n) is indicated. Scale bar = 5 μm. (C) Samples were analyzed by Coomassie blue staining. (D, E) Western blot using the antibodies indicated on the left. (C,D) bacterial supernatants; (E) bacterial pellets.