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. 2015 Jul 1;10(7):e0131216. doi: 10.1371/journal.pone.0131216

Fig 2. The proteolytic activity of plasmin increases the phagocytosis of microparticles, but not nanoparticles, by MoDCs.

Fig 2

MoDCs were treated with 500 nm fluorescent microparticles or 40 nm fluorescent nanoparticles in the presence/absence of 100 nM active or inactive plasmin. 3 h later the relative number of particle-positive MoDCs (Panel A) and internalized particles (Panel B) were assessed by flow cytometry. Data shown are mean ± standard error (n = 3–4 independent experiments). Data were normalized to the groups where MoDCs received neither plasmin nor inactive plasmin. *p<0.05 by 1-way ANOVA with Newman-Keuls post-hoc analysis. (C) MoDCs were treated with 500 nm fluorescent microparticles in the presence/absence of 10 nM active plasmin and/or 10 mM EACA. The relative number of particle-positive MoDCs was assessed by flow cytometry 24 h later. Data are displayed as mean fold-change in particle positive MoDCs ± s.e.m. (n = 4 independent experiments). Data was normalized to MoDCs receiving neither plasmin nor EACA. *p<0.05, **p<0.01 and ***p<0.001 by 1-way ANOVA with Newman-Keuls post-hoc analysis.