Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 May 15;6(2):252–266. doi: 10.3390/genes6020252

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Geminin^AWA does not inhibit re-replication. Replication extracts made from Xenopus eggs were depleted of both Geminin and Cdt1 using specific antibodies, then supplemented with recombinant Geminin:Cdt1 complexes. Replication was allowed to progress in the presence of BrdUTP, then the density of the product was determined by equilibrium ultracentrifugation. (A–D) Typical gradient profiles; (E) extent of re-replication for different Geminin mutants. The gray bar indicates the range, and the horizontal line indicates the mean. n, number of experiments.