Figure 3.
Effect of zearalenone and its metabolites on the cytokine synthesis in IPEC-1 supernatant. Cytokine synthesis was assessed in cell supernatant after 24 h of treatment with toxins at 37 °C. Data are means from four independent experiments. Two way ANOVA tests were performed to determine the effect of toxin treatment. Results are expressed as mean ± SEM for four different experiments, which are expressed as cytokine concentration (ng/mL). a–c indicate significant differences (p < 0.05) between control and ZEN and metabolites treated groups.