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ClustalW multiple sequence alignment of DON-Affinity binders with TRI101 enzymes/isoforms reveals some key amino acids mainly glycine, prolines, and glutamines (noted by under-stars) that are possibly important for DON binding/caging in addition to the highly conserved HXXXD motif (double-headed arrow). The catalytic residues of TRI101 reported by [62] are indicated by circles.
