Table 3.
TF binding sites that are commonly present in the promoters of 5 up-regulated genes tested by the NF-κB Signaling Targets PCR Array, predicted by TFSEARCH (threshold 85.0). The presence of these TF binding sites is shown for 4 down-regulated genes when cells were treated with venom and LPS. “x” represents the presence of the respective TF site in the promoter region of that particular gene. At the bottom, the fold regulations are presented for the selected genes.
| NF-κB signaling target genes | Cd83 | Csf1 | IL15 | Irf1 | Icam1 | Il1b | Csf3 | Mmp9 | Ccnd1 |
|---|---|---|---|---|---|---|---|---|---|
| TF binding site | |||||||||
| NF-Kap | x | x | x | x | x | x | x | x | x |
| C/EBP | x | x | x | x | x | x | x | x | - |
| C/EBPa | x | x | x | - | x | - | x | x | - |
| AML-1a | x | x | x | x | x | x | x | x | x |
| CdxA | x | x | x | x | x | x | x | x | x |
| CRE-BP | x | x | x | x | x | x | - | - | - |
| deltaE | x | x | x | x | x | x | x | x | x |
| GATA-1 | x | x | x | x | x | x | x | x | x |
| GATA-2 | x | x | x | x | x | x | x | x | x |
| GATA-3 | x | x | x | x | x | - | x | x | x |
| GATA-X | x | x | x | x | x | x | x | - | x |
| HSF2 | x | x | x | x | x | x | - | x | - |
| MZF1 | x | x | x | x | x | x | x | x | x |
| Nkx-2. | x | x | x | x | x | x | x | x | x |
| Oct-1 | x | x | x | x | x | x | x | x | - |
| SRY | x | x | x | x | x | x | x | x | x |
| TATA | x | x | x | x | x | x | - | x | x |
| Fold regulation | |||||||||
| venom- and LPS- vs. LPS-treated | 29.4 | 117.8 | 13.4 | 14.0 | 17.6 | −4.4 | −31.7 | −5.8 | −7.4 |
| venom-treated vs. untreated | 3.0 | 55.9 | 2.5 | 2.0 | 1.2 | 5.9 | 5.5 | 1.6 | −2.7 |