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Circular dichroism spectra of Stx2c/c and Stx2d/d. Mean residual ellipticitities (MRE) are plotted as a function of wavelength. Spectra of native Stx2c/c and Stx2d/d were acquired at 20 °C. Post exposure to heat (PEH) spectra were acquired at 40 °C. Secondary structural analyses of both native and PEH toxins were done as described in the Experimental section, and the results are presented in Table 2.
