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. 2015 Jun 11;7(6):2980–2998. doi: 10.3390/v7062756

Figure 3.

Figure 3

IFN responses upon inoculation with rNDVs. Cells were either mock inoculated or inoculated with the indicated rNDV at m.o.i. 3. After 24 h: (a) RNA was isolated and quantitative real-time polymerase chain reaction (qRT-PCR) was performed for endogenous hIFNβ mRNA. Results are presented as fold change gene induction of treated versus mock treated cells calculated using the 2−ΔΔCT method [31]. Means and ranges of triplicate experiments are plotted; (b) Supernatants were tested for functional IFN protein content using an ISRE-luc bioassay [14]. Results are presented as fold change in luminescence compared to mock inoculated cells. Means and standard deviations of triplicate experiments are plotted. x = p < 0.05 vs. MRC-5 (one-way ANOVA + Bonferroni post-test), * = p < 0.05 vs. rNDV-F0 (one-way ANOVA + Bonferroni post-test).