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. 2015 Jun 12;7(6):3019–3034. doi: 10.3390/v7062758

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Schematic representation of the recombinant constructs and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)analysis of purified proteins. (A) Schematic of the recombinant phage endolysins used in this report. The fusion construct PlyGVE2CpCWB consists of the amidase domain from the PlyGVE2 endolysin (light grey) and the cell-wall binding (CWB) domain of the PlyCP26F endolysin (dark grey); (B) Representative 15% SDS-PAGE of the endolysin constructs. Lane 1. PlyGVE2CpCWB, Lane 2. PlyCP26F, and Lane 3. PlyCP39O, M = markers (Precision Protein Plus, Biorad). All proteins are nickel column purified at >95% purity and the purified protein full length amino acid sequence was further verified by mass spectrometry (See Supplementary Information).