Table 1.
Primers used for Israeli acute paralysis virus (IAPV) detection, for virus quantification and for dsRNA synthesis, T7 promotor-sequence not included.
| Target | Use | Forward Primer (5′→3′) | Reverse Primer (5′→3′) | Reference |
|---|---|---|---|---|
| IAPV | PCR | CGAACTTGGTGACTTGAAGG | GCATCAGTCGTCTTCCAGGT | [30] |
| PPIA | RT-qPCR | TCGTAATGGAGTTGAGGAGTGA | CTTGGCACATGAAGTTTGGAAT | [31] |
| IAPV | RT-qPCR | CCATGCCTGGCGATTCAC | CTGAATAATACTGTGCGTATC | [31] |
| dsVP586 | dsRNA | ACCTGGAAGACGATTGATGC | CTGCCCACTTCCAAACAACT | This study |
| dsVP443 | dsRNA | TATAGATGCCGCTCCATGTG | CTGCCCACTTCCAAACAACT | This study |
| dsVP293 | dsRNA | ACCTGGAAGACGATTGATGC | GTGGGTTTGACGGGTATCAC | This study |