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. 2015 Jun 23;7(6):3329–3344. doi: 10.3390/v7062774

Figure 5.

Figure 5

Putative translation mechanism and proteins maturation of ORFs 2 and 3 on CBPV RNA 2. Top: Diagram of predicted genome organisation of ORFs 2 and 3 on CBPV RNA 2. The ORFs are indicated with their positions and putative amino acid sequence length. aa: amino acid, pSP: predicted structural protein, hSP: hypothetical structural protein; (A) Putative fusion protein capsid of about 65 kD resulting from translation initiation from the first ATG of ORF 3 and recovery of ORF 2 around the STOP codon of ORF 3; (B) The p65 may be cleaved by means of two non-simultaneous cleavage sites (arrowheads) into two proteins of about 45 kD, pSP until mid of p65 and mid-C-terminal region of p65 corresponding to immunodetected protein about 44 kD; (C) The immunodetected protein of about 28 kD may be pSP alone with post-translational modifications; (D) The products of maturation of about 44 kD may be cleaved into several proteins corresponding to immunodetected proteins at about 18 kD.