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. 2015 Jul 2;9:152. doi: 10.3389/fnbeh.2015.00152

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Copyright © 2015 Gompf, Budygin, Fuller and Bass.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Adeno-associated virus (AAV) transgenes used in this study. Schematic maps of AAV vector constructs. (A) Effector gene constructs, ChR2 and hM3Dq and the promoters used to drive high neuronal expression rates, EF1-α and hSyn, respectively. Note that both effector molecule sequences are in the DIO position. (B) Maps of the three vector constructs used to drive expression of Cre-recombinase. Sizes are approximate and demonstrate that the TH promoter is much larger than either EF1-α or CMV. (C) Unlocking of the hM3Dq construct from the DIO orientation to the sense orientation following Cre-mediated recombination.