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. 2015 Jul 2;9:152. doi: 10.3389/fnbeh.2015.00152

Figure 7.

Figure 7

Dual injections to either the LC or an LC projection. (A) Dual injection of CMV-Cre-AAV and hM3Dq-AAV into the LC results in widespread expression of hM3Dq throughout the pons. (B,C) Widespread c-Fos expression throughout the pons (B) as well as the cortex (e.g., ACC, (C) was observed following CNO administration. (D) Wakefulness is increased in animals receiving this dual injection following CNO administration (blue line), whereas neither saline in these animals (red line) nor CNO injections in animals that did not receive viral injections (dashed gray line) produce prolonged wakefulness. (E,F) WGA-Cre-AAV injection into the ACC results in Cre expression in the ACC (E), as well as transport of Cre to the LC (F), where the AAV-hM3Dq vector was injected. (G) cFos expression is lower in these animals than in animals expressing hM3Dq throughout the pons (B,C). Wakefulness (H) in these animals is greater following CNO injection (blue line) than saline (red line).